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In this context, the location of RTN4R within the replicated and highly penetrant 22q11.2 schizophrenia susceptibility locus makes the gene a prime candidate for influencing disease susceptibility.
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Main results showed that larger subsample areas produced higher and more accurate CFR values, mainly related to higher srichness values and to smaller variability within the replicates.
First, normalization within the replicate is performed.
For sppp, a high value indicates consistent overdispersion within the replicate datasets.
High-correlation coefficients for peak intensity within the replicates were observed (Supplementary Figure S2).
Considerable inter-tumoural heterogeneity within the replicate samples was observed, consistent with our previous studies [ 11].
The coefficient of variation (CV%) within the replicates was less than 12% for all the analyzed samples (Fig. 1D).
Coefficient of variation (CV) was calculated from replicate values within the replicate plates and mean values between replicate plates (Kinnear and Gray, 2007).
The same volume of culture added to the common pool from each replicate was then transferred from the common pool to each microcosm within the replicate [ 17].
Low p-values can result from a high technical variation within the replicates or reflect the biological truth within the samples.
We found that the latter is just slightly higher than the former, which means that the variation among genes is slight higher than the variation within the replicates of the same genes (lowest ratio is found for adults samples: 1.1).
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