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Twenty-six genes (the number of genes within the relocated region) were drawn randomly from the set of all genes within the nonrecombining region.
If the substitution rate was the same between these chromosomal regions, the suppression of recombination within the relocated region would have had to occur at an evolutionary time point that was more than twofold earlier than the other rearrangement events.
The median Ks values between the N. tetrasperma mat A and mat a strains for the genes within the relocated region are ∼2.7 times greater than those for the next most divergent regions.
Ka permutation tests were performed similarly except the median Ka of randomly sampled N. tetrasperma mat A and mat a ortholog pairs was compared to that of the genes within the relocated region.
Each gene's CAI was subtracted from that of its ortholog in N. crassa and the median of the differences was calculated for the random sample and compared to that of the real data (i.e., the genes within the relocated region).
Surprisingly and counter to our model of the order of rearrangement events, we found that the Ks values for genes within the relocated region were much larger than those for genes within the 5.3-Mb inversion (Bonferroni-corrected Mann-Whitney U (MWU) test: P = 4.33 e-06; Figure 3).
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These sequence features also support the inclusion of the relocated region within both inversions, providing additional support for our model of how this region moved from one end of the chromosome to the other.
Gray dots denote the hypocenters of the relocated fore- and aftershocks within 5 km from the fault model.
Black spots denote the relocated hypocenters within a horizontal range of 10 km from the cross section.
Additionally, significant numbers of people have moved within the country, relocating from malaria-free areas to malaria-endemic areas due to resettlement or economic emigration.
Finally, Table 2 shows that the length of time that one has been using the LRT system is not significantly associated with the propensity to have relocated within the prior 5 years (t = 1.51), though is positively associated with the distance between [new] home and LRT station.
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