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Between the 11 computer predicted fragments (lane 6) and 10 experimentally amplified fragments (lane 5), eight fragments matched within the length range of 100 1500 bases (Figure 4).
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Because the lengths of plant pre-miRNA are usually between 60 ~ 500 nt, we selected the introns within this length range for secondary structure prediction using RNAfold [ 82].
If both elements were within this length range, we randomly chose one element.
While more than 96% of all the cells were within the same length range as PCC 7942, some cells were up to 26% larger than WT.
However, a number of differences remain noteworthy: (1) Despite equal GM relative lengths within the overlapping length range, corresponding moments exerted at the ankle differ substantially (marked for reference in Figure 5B).
Since TaqMan probes designed for real-time PCR are usually within the above length range, we conclude that regular TaqMan probes can be used directly for FMCA.
These values fall within the length variation range reported for other metazoan mitochondrial genomes.
The complete mtDNA sequences of A. planci and A. brevispinus are 16,234 bp and 16,254 bp in size, respectively; both fit within the length variation range reported for other metazoan mitochondrial genomes [ 9].
Of the 327 SSR markers found to amplify alleles within the prescribed genotyping length range (100 700 bp), 283 (87%) were polymorphic among IB25, IB72, and IF74.
Length analysis of 3′UTRs revealed that 41.8% and 38.2% of bovine and feline sequences, respectively, were found within the 51 – 200 nt length range.
A measurable frequency of TRD has also been detected at telomeres within the wild type length range, suggesting that TRD can also function as a telomere length regulator [37].
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