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This value is within the expected sequence variation of mt sequences of different individuals.
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As expected, sequence conservation is seen primarily within the aligned granulin domains of the zebrafish precursors.
Hydroxyproline was only observed in the proteins known to have it (collagen and proteins containing collagen domains; emilins, etc) and only within the expected GXPG sequence motifs.
To determine whether the plasmids contain our expected sequences, we digested the plasmid with restriction enzymes specific for the 10 segments within each array.
We excluded ants in this analysis because we wanted to test whether SSOG counts observed in these recently sequenced genomes fall within the expected range based on previously sequenced insect genomes.
Sequence analyses confirmed the expected DNA sequence.
Through amplicon melt analysis and direct sequencing of the 235-bp PCR-amplified fragment, the region corresponded to the expected specific sequence within the D1/D2 LSU rDNA domain, according to Hosoi-Tanabe and Sako (2005).
However, miRNA length distribution analysis (Fig. 1) showed the highest count of miRNA-associated reads was 22 nt in length (40.14 %), and the majority of unique sequences reads ranged within the expected length of 21 23 nt.
Other genes within S. cerevisiae that had not been assigned an ortholog were further analysed with the hypothesis that these may have exonerate hits within the expected positions but were not the most similar sequence within S. arboricolus.
We expect sequence signatures comprising potential secretion signals and host-translocation motifs to occur within the 150 N-terminal residues of a protein.
In order to remove those, all sequences longer than 1,550 bp were aligned to the remainder of the sequences in the database (those within the expected length of an SSU rDNA gene), using BLASTN.
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