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Peaks within the expected mass ranges from the ICE and LEA lines are indicated.
Only in two of the transgenic lines, we found a peak within the expected mass range of the expressed peptides for ICE – 4,215.85 Da (calculated monoisotopic mass 4,213.92 Da) and LEA – 9,122.71 Da (calculated monoisotopic mass 9,119.53 Da).
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The intensity of the peak with the smallest deviation from the expected mass within a 10 ppm window was summed for each potential partner over the same MS range as the light species.
Neutral loss fragments were identified by parsing MS2 spectra for fragments within 0.2 m/ z of the expected mass (based on precursor m/ z and charge state) and with an intensity greater than 10% of the base peak intensity.
The measured molecular mass of the WT-Tm dimer was 65676.04 Da compared to the expected mass of 65675 Da.
These molecular mass values correspond well to the expected mass of the dADA2bL and dADA2bS isoforms, respectively.
The masses of both heterodimers were within 0.9 Da of the expected masses.
The expected masses were calculated from the masses of utilized sugar.
These corresponded to the expected masses with 0.07% of mass accuracy.
Mass spectroscopic analysis indicated the protein to be 17.5 kDa, which equalled the theoretically expected mass.
The predominant peak for 4YSyn by MALDI-TOF analysis was 6592 m/z, which corresponded to the 6718 expected mass of purified recombinant α-Syn within <2% mass accuracy (Figure 3D and Table 3).
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