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When Gag-miRNA complexes are present within the assembling complexes, particle formation is disrupted, resulting in internalization of misassembled complexes through endocytosis.
This mechanism would explain increased Gag processing, as enhanced lipid raft-binding and Gag multimerization would in turn facilitate dimerization of Gag-Pol precursors within the assembling virion [92] and thus activation of the viral protease.
Within the assembling profile, the slope of the transitions directly correlates with Δ G°, as the steeper slope results in more negative Δ G° values.
Less apparent is the role that the dual interactions play in facilitating the localized folding events within the assembling ribosomal subunits.
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A particular issue in the current assembly is duplicated sequence, both within the assembled chromosomes, and between sequence on the assembled and unplaced scaffolds.
Our approach identified many TEs not found within the assembled genomes, confirming the presence of collapsed TE sequences in our assemblies and providing a different perspective on TE content across the phylogeny (fig. 2 B).
For example, with a threshold of 60% the procedure stops, if the patients within the assembled total ZIP code area cover 60% of the HS's patient volume.
In addition, the cross-linking screen identified six positions of intramolecular cross-linking within the assembled protein but not in the monomeric S-layer protein.
Importantly, nanofibers within the assembled microfluidic channels were robust and did not break or wash out of the channel under extreme fluid flow conditions at linear velocities up to 13.6 mm/s.
The present results clarify the key role of the gp5 Δ-domain in prohead I architecture by providing direct evidence of domain structure stabilization and interdomain interactions within the assembled shell.
Probe sets with consensus alignments that mapped to a single genomic location within the assembled chromosomes were retained.
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