Exact(41)
Before the protein quantification, the samples were diluted in the appropriate buffer so that the concentrations determined would fall within the assay and equipment limits.
The inclusion of a third enzyme, glucose dehydrogenase (GDH), provided cofactor recycling to enable low concentrations of NADPH to be incorporated within the assay.
Cut point determination is described within the assay validation section.
The results were found to be within the assay variability limits during the entire process.
Departure from HWE was used as an indicator of genotypic class bias within the assay and was measured with a χ2 test.
For each Epityper assay, a median CV of all the CpG units within the assay was also calculated.
Similar(19)
Confocal images displaying the localization of pockets filled with the assay within the polymeric matrix are presented in this paper.
Reproducibility within the assays was evaluated in three independent experiments.
Cross-interactions between the assays and cross-reactivities within the assays were examined.
The lower than expected reproducibility might be due to increased number of SNPs in the assay and interactions with other oligonucleotides within the assays.
To that aim, we determined the method that best discriminated between the compounds representing positive and negative controls within the assays.
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