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For this, MCF-10A, MCF-7 or MDA-MD-231 were cultured on 2D conditions, or within staged 3D ECMs for a period of 18h.
Real time motility (on control 2D), as well as invasive (within staged fibroblast-derived 3D matrices) 6 h period assays, were used to observe correlations between invasive behaviors and substrate-induced morphologies of the cells used in this study.
For this, MDA-MB-231 cells were plated overnight within staged 3D ECMs in the presence or absence of Wortmannin and/or mAb13 (DMSO and/or rat antibody were used as negative controls; see Methods for details).
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The 5-year survival rate was 88% for stage II+ breast cancers detected at the prevalence screening, suggesting a within-stage lead time.
Within-stage amplified cDNAs were pooled in equimolar amounts (as estimated from agarose gels) before the DNA digestion step that precedes insert ligation into the λTriplEx2 phage arms.
No other pairwise comparisons of mean number of interactions per gene were statistically significantly different, including both within-stage comparisons of gonadal to non-gonadal tissue.
The first is staged within an active construction site near the gallery.
The two-sided show is staged within the London offshoot of Galerie Artima. .
Other radical features of Cunningham dance theater included highly unconventional rhythm, the placing and focusing of dancers within stage space and a pronounced degree of ambiguity.
The decision to merge across or within stages of production is treated as endogenous.
We found that the daily activity area (mean 4.4 km2) did not vary within staging periods.
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