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Replicates were included, including four duplicates that were included on both arrays and five duplicates that were included within one array.
Representing the mismatch tallies of a whole cohort within one array allows for convenient analyses across positions and samples.
The spacer sequences are 26-72 nucleotines in length, where the variance of spacer length within one array is small.
Technical and biological repeats were compared after averaging data from duplicate spots within one array, which produces more accurate measurements and reduces variability.
Our analyses indicate that DXZ4 monomers within one array are more similar to one another than they are to monomers in an array from a different individual.
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The signals from one array deviated considerably from its biological replicates within the heart group, reflecting technical difficulties noted during hybridisation, and therefore this array was excluded from further analyses.
Gene expression data from one array were excluded from further analysis due to a within individual cell line linear r2 correlation for all genes of <95%, while all remaining within individual cell line correlations were ~99%.
Four transducers were compared using these protocols (two curvilinear arrays, one phased array, and one two-dimensional array).
One component of the yeast spindle pole body, Spc42p, has been found to form a crystalline array within one of the central layers of the structure; the Spc42p crystal might provide a scaffold around which the spindle pole body is assembled, and could be involved in regulating the size of the spindle pole body.
Across the arrays, within one category, the target and foil items move around to different positions; furthermore, 2 of the 10 items in each category are never targets.
The TMA was constructed from duplicate 0.6-mm tissue cores that were removed from the central portion of a representative paraffin block from each tumor and arrayed within one of seven paraffin blocks, using a tissue arrayer (Beecher Instruments, Inc., Sun Prairie, WI, USA).
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