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Detection range for creatinine was 4 - 1238 μmol/l and within lab coefficient of variation was 1.1%.
To further explore attributable variability, we performed a discriminant analysis on the following factors: gender, diet, strain, fasted, and coded study (nested within lab).
Considering wild-derived and lab-derived strains independently, principal component analyses (PCA, an unsupervised linear feature extraction method that discovers the directions of maximal variances in data) found highly significant correlations between non-synonymous SNP distribution and phylogeny within lab strain V2Rs (Two-way ANOVA, variance by V2R clade F12,858 = 17.99, P < 0.0001).
These genes were acquired from a diverse group of non Lactobacillus/Pediococcus Gram-positive microorganisms; however, the most common source was Entercococcus, suggesting that this genus, likely due to its promiscuous nature, has played a significant role in the exchange of genetic material within LAB.
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These data suggest that our procedures were effective at reducing within-lab variability.
The repeatability and within-lab reproducibility were calculated using single factor analysis of variance (ANOVA).
The within-lab reproducibility was determined at the CCβ concentration level.
The resulting standardized method is then evaluated for within-lab and between-lab reproducibility and for its accuracy.
The null hypothesis that all within-lab variances are equal could be tested against the general alternative.
Good linearity, trueness, repeatability and within-lab reproducibility were obtained for zanamivir, ribavirin, oseltamivir, oseltamivir carboxylate, amantadine and rimantadine.
Accuracy, intra-day precision (repeatability), inter-day precision (within-lab reproducibility, muscle tissue only), recovery, linearity, CCα and CCß were determined.
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