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For each plot, we see that within each platform, the distributions of different profiles (without normalization) show similar patterns.
We examined microarray data for consistency within each platform by reviewing the repeatability at two levels: the quantitative signal values and the qualitative microRNA list agreement.
Detection in each tissue was defined as detectable in 3 out of 4 technical replicates within each platform.
These two metrics did not reveal noticeable differences between the two mouse retina mRNA pools within each platform.
Technical replicates were assessed both within each platform and between two platforms to examine reproducibility and consistency.
The results of the Rankprod analysis, for those miRNAs present in all platforms, are shown as a heatmap of color-coded ranks of log ratios within each platform.
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Relative performance among platforms was consistent with the results reported above, which are based on data normalized to 100× mean coverage within each platforms target region.
We believe TestFlight has done a great job simplifying a complex process, but we have to work within the rules of each platform.
Lastly, it should be recognized that these technologies measure relative expression within the context of each platform.
For the sake of consistency in the comparison, two test sites were chosen at random and combined for each platform within the MAQC dataset.
The technical precision of each platform, within the same hybridization experiment or qPCR plate, was also calculated for different copy number levels based on data from duplicate arrays or triplicate qPCR reactions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com