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Pre-treatment and post-treatment cases and controls were randomised across batches and within each batch across beadchips, and included an average of 29 cases and 17 healthy samples for batches 1 9.
Based on the assessment of technical artifacts using principal component analyses, a plate effect was observed within each of the three batches and a chip within batch effect for the HumanMethylation27 data (Additional file 1: Figure S1).
Likewise, the duplicate samples illustrate the reproducibility of the dust preparation and analytical methods within each sample batch.
Within-batch and between-batch imprecision (assessed by the coefficient of variation) were better than 5.6%% for all analytes.
Seradyn internal teicoplanin controls were run within and between each batch.
Each reader had a high degree of within- and between-batch reliability (for example, intraclass correlation (ICC) (95% CI) for within-batch reliability for percent density varied between 0.94 (0.90, 0.98) and 0.97 (0.99, 0.99) across the various readers, whereas ICC for between-batch variability varied between 0.90 (0.84, 0.97) and 0.97 (0.99, 0.99)).
For quality control, samples from two sera were analyzed in duplicate within each analytical batch.
Every batch is sold out within an hour, and the price point ($5.00 each) is five times higher than the generic glazed doughnut down the street.
Within- and between-batch coefficients of variation were <10% for all assays.
The total (within- and between-batch) coefficients of variation were <20%.
Within- and between-batch precision values were lower than 3% [ 19, 22].
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