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The effect of ambient temperature on total recess time, recess frequency and average recess length was investigated at the level of clutch, per day nested within clutch and per hour nested within clutch and day.
To analyze pre-hatching viability, I nested egg (N = 155) within clutch (N = 69) and included the independent contrasts for laying order as predictors.
Nonetheless, I analyzed egg volume, as one component of investment, with egg (N = 156) nested within clutch (N = 70) as a random intercept and with the independent contrasts for laying order as predictors.
While in the control de-chorionated embryos the mortality rate was 4 9 per 100 embryos, cocaine treatment increased this rate 6±2.1% and 37±6.3% above control level following 1.5 µM and 30 µM dose, respectively (p<0.05 for both, based on paired t-test of within clutch comparisons, six individual clutches per treatment).
Within clutch, eggs were sired by one or two males in variable proportion.
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Riehl, C. Infanticide and within-clutch competition select for reproductive synchrony in a cooperative bird.
Further, the common eggshell microstructure and within-clutch egg pairing12 in these dinosaurs, as well as an oviraptorosaur adult with two internal eggs13, indicate that overall ovary and oviduct function in these dinosaurs matched those of modern birds in producing eggs iteratively at daily or greater intervals, but from two active reproductive tracts12,13,14.
We quantified within-clutch size variation at hatching and found that newly hatched larvae from the periphery (5 mm from edge) of 2-dimensional clutches were smaller in standard length, cranial depth, eye diameter and body area (7%, 8%, 4% and 11%, respectively) than larvae from the interior positions within clutches.
Since different clutches can show substantial differences in survival rate, presumably due to differences in egg quality from different zebrafish parents, paired within-clutch comparisons were used.
The results based on the within-clutch comparisons, e.g., for mortality rate change in six clutches, were analyzed using paired t-test.
Even if within-clutch variation in hormone deposition would occur, the fourth egg would still represent the medium concentration of the whole clutch, as long as the pattern of within-clutch variation is linear (as found in many studied species with significant within-clutch variation, e.g. Reed and Vleck 2001; Groothuis and Schwabl 2002; Pilz et al. 2004; Tschirren et al. 2004).
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