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When included in analyses, ASSAY was fitted such that LINE was nested within ASSAY.
Repeated analysis of the kit's control samples showed good precision (coefficient of variation (CV) of 3% within assay and 10% between assays).
Within assay precisions and accuracies (bias) were 3.4 21% RSD and from −24 to 21% for the concentration range 1.00 5.00 μM, respectively.
Interestingly, the HA1 globular domain protein generated similar titers of neutralizing antibodies with the same profile of cross-clade neutralizing activity as was observed for the intact HA0 protein (two-fold difference in titers falls within assay variability).
For both the M30 Apoptosense® ELISA and M65® ELISA, within assay (WA % CV) variation was <10% and between assay (BA % CV) variation was <10% for samples >100 U/L.
The within assay and between-assay coefficient of variation are both less than 5%, respectively.
Similar(44)
The effect of each successive filter on the within-assay pairwise similarity is shown in Fig. 2.
Table 3 Precision of the proposed assay at different CLM concentrations Concentration (μg mL−1) Relative standard deviation Within-assay, n = 5 Between-assays, n = 5 50 1.25 1.82 250 0.98 1.05 500 1.34 1.65.
Table 3 Precision of the proposed assay at different OLM concentrations Concentration (μg ml-1) Relative standard deviation Within-assay, n = 5 Between-assays, n = 5 30 1.55 1.55 60 1.55 1.55 1.551.55 1.55.
All samples from one participant were analysed on the same ELISA plate; the within-assay coefficient of variation for the analyses performed was as follows: adrenaline 2.7%, pCort 1.3%, pTest 4.5%, sCort 2.8%, sTest 3.7%, sCgA 5.2% and sAA 4.9%.
Table 2 Precision of the proposed assay at different ATR-Ca concentrations Concentration (μg/well) Relative standard deviation Within-assay, n = 5 Between-assays, n = 5 25 1.11 1.17 50 1.17 1.17 1.171.17 1.17.
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