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This biosensor consists of three parts, a freeze-dried biosensing strain within a vial, a small light-proof test chamber, and an optic-fiber connected between the sample chamber and a luminometer.
For statistical analyses, comparisons between experimental groups were made on the totals of feeding events by all flies within a vial, to avoid pseudoreplication.
The distribution of total abundance is approximately lognormal; it is not uncommon to find flies in the same vial whose total abundance differs by a factor of 2. However, although individual fly TA varies greatly within a vial or condition, the population distribution of TA values observed is reproducible within one condition.
The whole of the sample volume within a vial in the aluminum module cooled uniformly below the equilibrium melting temperature of the solution before ice nucleation occurred and solidification then progressed instantaneously and in a relatively uniform manner throughout the cryovial, with no large temperature gradients being observed (Fig. 4 – right).
Since this vaccine was new to the country and has new packaging system (preservative free two doses with in a vial; the standard and other countries using is single dose within a vial), EMoH with its partners were conducting survey in selected sites of the country.
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The 7 sec observation time was chosen because preliminary assays showed that all unstressed flies reach the top of a vial within 6 sec (5.1±1.3 sec, n = 50).
Specimens from 12 cardiac valves positive on polymerase chain reaction (PCR) for Bartonella quintana or Brucella melitensis were spread onto cells grown within a shell vial as previously described (13, 14 ).
"They learn to cork their anxiety within themselves like acid in a vial.
Starting at the end of 2010, the meningococcal A (MenA) vaccine (MenAfriVac) has been introduced through phased mass campaigns in the high-prevalence meningococcal belt in West Africa., (MenAfriVac presents in a 10-dose lyophilized vial and needs to be preserved at temperatures between 2°C 8°C. Once open, a vial should be used within 6 hours).
The external signal can be acquired from a vial placed outside the subject but within the sensitive volume of the RF coil [52] [57].
Counting of eggs was greatly facilitated by placing egg masses in a vial containing 70% ethanol, which renders the egg bodies opaque within an hour.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com