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To assess co-expression perseveration between the species on a module-by-module basis, we first calculated a value of the module membership (MM) - a measure of how well each gene correlates with the first principal component of gene expression within a module, termed as the module eigengene.
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The initial module, termed the Small Portable Expeditionary Aeromedical Rapid Response (SPEARR) team, consists of 10 crew members with equipment that can fit within a medium-size trailer.
The tool uses a Fisher's Exact test to look for significant over-representation of terms within a module versus the TAIR10 genes represented on the ATH1 platform as background.
Only terms enriched within a module with a Fisher's p-value of 0.05 or less were considered.
Genes with the highest degree of connectivity within a module (centrally located genes of co-expressed genes) are termed hub genes and are expected to be drivers required for signaling pathways of essential cellular function.
To determine the enriched GO terms of genes within a module, we conducted GO enrichment analysis using the GO Term Finder package [37].
We believe the higher density of the modules when compared with either size-equivalent random networks or with the entire network, the GO-term enrichments found in each module, their functional specialization (few shared GO terms between modules), and their functional homogeneity (similar or related functions within a module) strengthen the biological relevance of our IRES predictions.
Most indices try to optimize the balance between tightness (the expression variability within a module) and separation (the expression differences between modules).
Apart from those parameters influencing the number of clusters, most module detection methods also have other parameters, frequently affecting the compactness of the modules, the way local co-expression is defined or the minimal number of genes within a module.
Each object within a module is labeled with a location (often including a street address) or with a reference to a page in the artist's multivolume encyclopedia.
The biological homogeneity index measures the proportion of gene pairs within a module which are also matched within a functional class22.
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