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The amount of gentamicin eluted at the first withdrawn sample ranged from 30% to 60% of total gentamicin released over the assay.
The same volume of fresh medium bubbled with CO2 was added after each withdrawn sample [ 30].
The withdrawn sample was replaced with equal volume of fresh buffer solution to keep the volume of release fluid constant.
Upon reaching a nitrite concentration of ∼5 mM, 5 mL of each culture was withdrawn and processed anaerobically as follows: each withdrawn sample was divided in four.
To make the sample pipettable equivalent to water, 1 g of the withdrawn sample from SSF was diluted five times with milliQ water.
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Encapsulation efficiency of withdrawn samples was determined.
A quantitative GC FID analysis of the withdrawn samples was performed.
The withdrawn samples were analyzed for drug content by spectrophotometer at 476 nm (Fig. 4).
OD, redox potential (Eh) and pH of withdrawn samples was measured instantly using EUTECH Instruments (pH and Eh Tutor).
The withdrawn samples were used for OD measurement at 237 nm by a UV-visible spectrophotometer (Shimadzu, model UV-2601).
All withdrawn samples from the reaction media during the process showed no antibacterial activity, which indicates safety of the treated effluent for disposal into the environment.
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