Slides were coverslipped with prolong gold antifade with DAPI, visualised with an Axioscope II with AxioCamMRC and analysed using Axiovision 4.6 (Carl Zeiss, Gottingen, Germany).
Single staining with CD163 was visualised with ultraVIEW Universal Alkaline Phosphatase Red Detection Kit Fastt red) detection system (Ventana Medical Systems).
The amplified DNA products were separated on a 2% agarose gel, stained with ethidium bromide, visualised with an ultraviolet transilluminator.
It allows users to investigate the genomic intervals together with the aligned reads and to work with, visualise and export these intervals.
Despite this, we were able to demonstrate significant differences between those with BMLs visualised only on T2-weighted images and those with BMLs visualised on both T1- and T2-weighted images.
Hierarchical clustering was performed using Cluster 3.0 [73] (Euclidean distance similarity metric with average linkage) and visualised with Java Treeview [74].
Immunoreactive bands were revealed with ECL Advance Western Blotting Detection Kit (Amersham Biosciences) and visualised with ChemiDoc ™ XRS System (Bio-Rad).
After washes, sections were covered with 50%% glycerol and stainings were visualised with a fluorescence microscope.
