Sentence examples for with two restriction from inspiring English sources

Restriction of genomic DNA with two restriction enzymes and ligation of double-stranded adaptors to the resulting restricted fragments were performed in one step in a thermal cycler (Veriti, AB, Life Technologies; 37°C for 2 h followed by a 30 min hold at 17°C).

The orientation of the subcloned fragment was checked by digestion with two restriction enzymes, Sal I and Xho I, sites that are present in the ORF of OsIDS1.

To confirm that the clones with homologous recombination at 5′ homologous arm do not harbor random integration, the genomic DNA was examined by Southern blotting with the upstream, downstream and internal probes, and with two restriction enzymes.

Total genomic DNA (200 ng) was digested with two restriction enzymes Pst I and Mse I and ligated to adaptors.

Similar results were obtained when the hybridization was performed with DNA samples digested with two restriction enzymes (NotI, SalI), although in this case two hybridizing bands were observed for BAC clone B57-6-5 B57-6-5 B57-6-5

Briefly, the genomic DNA was digested with two restriction endonuleases, EcoRI and HindIII, respectively.

The figures were compared to normal gene sets randomly selected with two restrictions (random set I, II, and III).

The amplified products of DNA from 80 Cronobacter strains were separately digested with three restriction endonucleases (Csp6I, HinP1I, MboI).

Dietary therapy, with methionine restriction and cystine supplementation (cystine is the oxidized form of cysteine) is apparently beneficial.

The Library of Congress collection was to be opened immediately after Frankfurter's death, with one restriction: no document could be seen until sixteen years had passed since the date it was written.

The synthesized MCS with seven restriction sites is sufficient for cloning 99.55% of the rice genes.