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The strength of the edges detected is also controlled with this detection system, which helps to enhance precision.
With this detection scheme, the average end-to-end BER is analytically determined in a closed-form expression with arbitrary number of relays.
The results show that the non-specific release of luciferase in the supernatant was barely detectable even in wells where the cell-associated luciferase activity was detected at an extremely high level (over 400,000 units, Fig. 3), indicating that non-specific release is not an issue with this detection method.
A detection range of 5 nM to 100 nM was achieved with this detection platform.
The strongest evidence that there may be issues with this detection method is their BLAST results for primers, which were independent of any laboratory work.
Less specific issues are also present with this detection technology: scanner saturation (signals may be stronger than the scanner's range upper limit), noise and variability of the measured signal as a function of the amount of spotted probe.
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Any reduction in recall due to the strand bias test was limited to SNVs with frequencies below this detection limit.
The 22 samples with the m.3243A>G heteroplasmic mutation revealed positive signals with both mutant- and wild-type-specific probes in this detection system with a detection limit of approximately 2%.
With this approach detection of single molecules are possible.
With this system, detection limits of similar magnitude to the ones reported in literature are reached.
With this method, detection limits of different gases could be determined, which lie in a range interesting for safety engineering.
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