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UROtsa cells were transfected with this constructed vector or a control vector using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's instructions.
The common Repository of Adventitious Proteins – cRAP (http://www.thegpm.org/crap/) was merged with this constructed database for searching (Protobothrops elegans plus cRAP = 116,501 entries; Protobothrops flavoviridis plus cRAP = 430,984 entries; Protobothrops hybrids plus cRAP = 237,041).
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Transformation of DGLA-producing A. thaliana with this construct yielded transformants that successfully produced prostaglandin F.
We present our experience with this construct failure and subsequent patient management.
With this construct in place, final reconstruction of the proximal femur around the stem is easier to achieve.
Mice vaccinated with this construct withstood challenge with as many as 1.5 million colony forming units of Y. pestis (7.7×104 LD50).
Intranasal vaccination of mice with this construct, without the use of adjuvant, resulted in 40% protection against challenge infection.
Immunization of mice with this construct yielded high IgG antibody responses without the use of traditional adjuvants.
We were able to demonstrate high levels of luciferase activity with this construct; however, selectivity was lost.
Phages prepared with this construct were analyzed as was described for M13-Cry1Ac. Figure 3a shows a phage blot assay showing that Cyt1Aa protoxin is displayed on filamentous phages.
Immunization of HLA-A∗0201 HLA-A∗0201 mice witransgenicnstruct efficiently elicited cellular responses to each intended epitope in vivo, further validating the applicability of this approach.
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