It is the result of an intense focus of mind and eye over many years, on a stretch of water, in a garden, planted with water lilies, and it invites the same concentrated communion in return.Monet's "Nymphéas" (1904), estimated by Sotheby's at £10-15m, represents a turning-point in the French artist's love-affair with his lily pond.
Those works contained the same concentrated power, but it appeared only in bursts, competing with design elements or interrupted by space-filling pure-dance sections.
Kevin Turner, Microsoft's chief operating officer, gets the same concentrated treatment.
Theres the same concentrated approach to using a simple strategy to build a big company.
We prepared a blank control solution for ICPMS reaction (without cells) by mixing 0.5 mL HBSS with the same amount of concentrated HNO3; this solution was processed the same way as the sample solutions.
In separate tubes, estrogenic compounds at nonsaturating concentration mediating about 80% MELN cell activity or 0.1% methanol extract for environmental samples, were prepared 4×-concentrated and preincubated with the same volume of 4×-concentrated purified recombinant ER-α(1 100 nM final concentration) in test culture medium at 4°C for 16 hr.
HUVECs were incubated in endothelial basal medium mixed with the same volume of 5-fold concentrated medium for cell proliferation, migration, and tube formation in vitro.
The enzyme sample was desalted and buffer exchanged with low ionic strength phosphate buffer (5 mM K-PO4, pH-7.2) using Zeba Desalt Spin Columns (Pierce) and further dialyzed for 48-h (12 hour periodic buffer change) with the same buffer at 4°C and concentrated using Amicon centriprep YM-10 centrifugal filter devices (Millipore).
For a fixed number of TMFD units distributed at fixed interval, the parameters can be optimized in a similar way as optimized for concentrated MTMFD system with the same governing criteria of minimization of peak mid-span displacement response of the bridge.
The concentrated protein solution was diluted 1 4 with the same buffer containing 100 mM NaCl and re-concentrated as above.
