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Fig. 3 Effect of illumination on the Skn7-dependent expression of the OCH1-lacZ transcriptional fusion (i.e. the mannosyl-transferase gene fused with the reading frame encoding β-galactosidase) in a S. cerevisiae strain with the wild-type sln1 gene, and in a strain with sln1 replaced by the gene encoding the C1-histidine kinase fusion protein.
All exon sequences were finally trimmed so that they started with the reading frame 0 and were a multiple of three nucleotides long.
This alignment determines the coding part together with the reading frame in the EST.
Potentially interesting are TRs in CDS regions where the unit size is not directly compatible with the reading frame.
This constraint may not be biologically realistic and would result in misaligned nucleotides when indels are not in phase with the reading frame.
For example, within the coding portion of the AtPLDα1 cDNA there are four regions with the reading frame shifted relative to the genomic sequence.
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The KIGs identified had between one and five domains within the reading frame, with the majority having two domains (61%%).
This type of error is identified and removed during our initial filtering steps, which eliminate reads with shifts in the reading frame.
The alignments were entered in the software Geneious Pro v5.5 [ 38] and a visual inspection was quickly performed in order to identify possible reads with changes in the reading frame.
In the ccsA gene, approximately 520 bp downstream from the start codon one or more deletions totaling 61 bp compared with V. minimum interrupt the reading frame.
In a protein-coding gene, the sequence of codons starting with AUG and ending with a termination codon is called the reading frame.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com