Sentence examples for with the dig from inspiring English sources

For double FISH, after hybridization with the DIG and Fluorescein labeled probes, binding of the HRP-coupled anti-DIG and anti-Fluorecein antibodies, the corresponding fluorescent TSA reactions were performed sequentially, inactivating the HRP activity with H2O2 in between the detection of the two antibodies.

The exhumations, conducted from August to October, removed 67 skeletons from the desert soil around Fort Craig — 39 men, 2 women and 26 children, according to two federal archaeologists who helped with the dig.

Mr. Kerasiotes has not said what he plans to do next, but in a recent interview he said he had given up plans to hold public office and expected to return to the private sector when his work with the dig was done.

Everyone is helping with the "dig out".

Hybridized RNA was detected with the Dig Luminescent detection kit (Roche) as described by the supplier.

Hybridization was done with the DIG luminescent detection kit (Roche Dignostics).

For Southern blot, genomic DNA of the transformants was digested by BamHI and hybridized with the DIG-labeled probes using standard protocols (Sambrook et al. 1989).

Sections of histopathology showed eosinophilic intranuclear inclusions (Cowdry type A inclusions or CAIs) in infected tissues while in situ hybridization, cells displayed an intense reaction with the DIG-labeled probe.

BamHI digested chromosomal DNA of the wild type and S. antibioticus ΔsimReg1 strains were probed with the DIG-labeled fragment containing simReg1, obtained as a KpnI fragment from the plasmid pKCEsimR1 Rebets et al. 2008.

Labelling was performed with the DIG-High Prime Kit (Roche, Mannheim, Germany) following manufacturers instructions.

We thank Dr. O.V. Okladnova for the generous gift of 6000-200 nt RNA markers, Dr. A.I. Kalmykova for her help with the DIG-hybridizations and photography of the samples and anonymous reviewer for helpful suggestions concerning the manuscript.