Exact(40)
We have defined the targeting of antigen directly to an APC as: the antigen co-localized with the APC (i.e. the color yellow), and/or the antigen in direct contact with the APC.
In order to utilize a relatively homogeneous system and to ensure that tumorigenesis was driven by 'second hits' at APC, we focused on individuals with known germline APC mutations, either humans with familial adenomatous polyposis (FAP) or mice with the Apc Min(R850X) mutation.
A major problem with the APC model is that it effectively shifts the costs of academic publishing from the reader to the author and therefore discriminates against those without access to the funds needed to meet these costs.
With the APC obtained at the optimum activation condition, the maximum adsorption capacity of 451, 362, and 320 (mg/g) was achieved for 2-naphthoic acid, diphenylacetic acid and cyclohexanepentanoic acid (CP), respectively.
The latter two proteins associate with the APC armadillo repeats.
Out of 319 detected ion peaks, 168 were identified with the APC components.
Similar(20)
Kit receptor was immunostained with the APC- anti-mouse CD117 antibody (eBioscience).
Mock- transfected cells incubated with the APC-conjugated anti-human Fc antibody were used as controls.
For BrdU analysis, cells were pulsed with 10 µM bromodeoxyuridine for 2 h and processed with the APC-BrdU kit according to the manufacturer's instructions (BD Pharmingen).
To identify a potent anti-Pol-β compound, we used an in silico high-throughput structure-based molecular docking approach and screened approximately 140,000 small molecule compounds (molecular weight <500 daltons) for their ability to interact with the APC-binding site of Pol-β (Fig. 1A) [48].
This also indicated that the Cdkn1a−/− 2D-DIGE targets were least supported by coexpression with the Apc-Cdkn1a network, with the p-values separated by two orders of magnitude again (p-value for coexpression of Cdkn1a−/− targets with differentially expressed network nodes was 3.3×10−5; p-value for coexpression of Apc1638N+/− targets was 3.1×10−7).
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