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To study events that are instructive for hair-cell rearrangements, we used chemical inhibitors to examine a candidate signaling pathway reversibly and with temporal precision.
Drosophila mushroom body neuroblasts (MBNs) are similar to mammalian neuronal progenitors in their capacity to generate several closely related neuron types that appear during development with temporal precision (Ito and Hotta, 1992; Lee et al, 1999).
All motor behaviors, in fact, require the coordinated activity of various muscles acting with temporal precision (Grillner, 2006, 2011), but the neural mechanisms underlying such temporal coordination are only starting to become clear.
Optogenetics is an approach to trigger gain or loss of function of explicit events in specific cells in tissue with temporal precision in the millisecond-scale, which is achieved through the use of light-sensitive control tools that may be targeted by gene delivery [ 1].
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At threshold levels of depolarization, EPSCs with short decay phase triggered spikes with high temporal precision, whereas EPSCs with a long decay time triggered spikes with poor temporal precision.
The other advantage of using street robbery as an example of criminal behaviour is that it is (usually) recorded with good temporal precision (personal communication with data provider).
In summary, with the average trajectories we can resolve the position and movement of different endocytic components in relation to each other along the direction of invagination with a temporal precision below 1 s and with a spatial precision of ∼10 nm.
Also, obtaining a global view of all the hydromineral parameters that the simulation can produce with equivalent temporal precision would be difficult or impossible to obtain with in vivo measurements.
"You could control, at precise times, a particular genetic locus and see how everything responds to that, with high temporal precision," says Deisseroth, who was not part of the research team.
The energy yields of a range of typical micro wind turbines (in the 0.4 2.5 kW size range) were estimated here using two wind speed datasets sited within 1 km of each other recorded with a temporal precision of 10 min.
Joint progress in light delivering approaches, multi-photon laser sources development, and opsins engineering has now brought the field of optogenetics into a new phase that we can name "circuit optogenetics", where neural circuits distributed between different brain areas can be optically interrogated and controlled with millisecond temporal precision and single-cell resolution.
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