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For plasmid targeting assays, the target plasmid DNA (4 10 mg/ml) with specified amounts of MgCl2 was injected into Xenopus laevis oocyte nuclei, followed within 1 min by the injection of RNPs (0.9 5.6 mg/ml), using different needles to avoid prior mixing.
The purpose of this study was to investigate whether Dutch food composition databases (Dutch-FCDB) are accurate enough to plan experimental diets with specified amounts of energy and macronutrients.
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Using interaction diagrams of axial force-bending moment, the value of safety factor for the critical section with specified amount of steel and concrete sections are computed.
For competition experiments, GST-SOX10 HMG protein was preincubated with specified amount of unlabeled probe for 15 min before exposure to the labeled specific probe.
The defect is then contaminated with 30 mg of sterile bovine collagen soaked with a specified amount of Staphylococcus aureus (S. aureus) in 0.5 ml of saline.
For activity assays, 1000 cpm of m7GDP, m7GpppG or m7GpppGm2′ were incubated with 1.5 µg of cellular extract for 60 min, or with the specified amount of recombinant protein for 10 min at 37°C in a 10 µl final volume.
First, it would provide seniors with a specified amount of money to purchase health benefits.
Acting techniques execute robot movements to supply the plants with the specified amount of water and nutrients.
These policies are complicated: benefits are triggered with a specified amount of medical problems, and there are limits to how much and how long policies will pay for your care.
Bruichladdich never figured out a cost-effective way to malt barley on the island all its barley is sent to a huge malting plant in Inverness, in the Scottish Highlands, which returns malt imbued with a specified amount of peat smoke.
"Someone recommended to me that we study the Alaskan model of revenue distribution back to the citizens and get bills introduced in state legislatures in California, Florida and elsewhere that if they go to offshore drilling they should split the revenue stream with a specified amount (maybe half) going to the citizens as a direct benefit," Mr. Gingrich wrote.
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