Sentence examples for with specific restriction from inspiring English sources

The final part of the method describes how specific PCR products when digested with specific restriction endonuclease enzymes, can be used to generate diagnostic DNA profiles (haplotypes) that can be used both to identify heterologous NPVs e.g. Autographa californica MNPV and related viruses, and to differentiate genotypic variants of Helicoverpa SNPV.

In brief, the crosslinked chromatin was sonicated, digested with specific restriction enzyme HindIII overnight, and immunoprecipitated with anti-SATB1 antibodies coupled to protein G beads.

The cDNA was PCR-amplified during in the initial 1 3 cycles using the specific primers to synthesize the dozens of gene-amplification products, each of which contained universal tags with specific restriction digestion sites for enzymes a and b at both termini.

Primers with specific restriction sites (KpnI, BglII or XhoI from New England Biolabs Inc).

Constructs were then sub-cloned into pCS2+ vector with specific restriction enzymes.

Restriction-enzyme-based reduced-representation sequencing: Genomic DNA is digested with specific restriction enzymes, followed by ligation of adaptors, amplification and sequencing.

The siting of the private stable in Manhattan is imperfectly understood; streets with specific restrictions against stables were few.

Professional cycling Although cycling could hardly be called exclusive, the sport is riddled with specific restrictions and inequalities.

Instead, Central Islip put in place a dress code with specific restrictions, including barring pajama-like pants, halter tops that expose the midriff, and T-shirts with inflammatory writing that expresses a message of violence or membership in gangs.

Being a bouncer is an actual job, with specific restrictions, targets and non-negotiable orders from bosses.

In many countries, genotype data are classified as sensitive data that can be handled by complying with specific restrictions, e.g. HIPAA in the USA and the Data Protection Directive in the European Union.