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However, 8.2 to 15.0% of the genes with significantly changes showed only subtle changes (fold change of less than 1.2-fold).
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The numbers of genes with significantly changed expression levels (fold change ≥2) between the corresponding conditions are shown in Fig. 2.
Using this approach we identified 32 protein kinases or phosphatases/phosphatase subunits with significantly changed protein levels in one or more training groups as compared to foot shock control.
Although the enhanced centrality of precuneus during the hungry state indicates a changed core hub of the default network, we found no other areas with significantly changed centrality values across the brain.
Comparisons between lists of contigs with significantly changed expression in the different treatments and the generation of preliminary Venn diagrams was performed using the FiRe macro in Excel® [59].
This is evident from the lists of contigs with significantly changed expression for each treatment relative to the control (Figure 2, Tables S1, S2, S3, S4, S5, S6) and is further highlighted by PCA (Figure 4).
Proteins with significantly changed abundance (FDR <0.1) are included in the table.
Transcripts with significantly changed expression (adj. p-value <0.05) are included in the table.
Genes with significantly changed mRNA levels were selected with a cut-off of P < 0.05 and fold change ≥ 2.0.
There were 5 muscle contraction genes with significantly changed expression in the diaphragm (4 increasedecreasedandd) and 6 with significantly changed expression in the sternohyoid (4 increasedecreasedased) with diabetes (Table 3).
Ten metabolites with significantly changed abundance were targeted for identification using MS/MS and comparison with chemical standards.
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