The association of the polymer with siRNA to form the polyplexes was observed by the retardation of siRNA bands on agarose gel electrophoresis.
Nanoparticle concentration measurements were quantified in a manner consistent with the protocol described by Bhise et al. (30) To evaluate the ability of the polymers to complex with siRNA to form nanoparticles, we determined the polymer/siRNA weight ratios (wt/wts) at which siRNA became completely complexed.
Since the induced expression of the Sec-deficient C-terminal mutant TR1 construct did not elicit an alteration in redox status of Trx, we evaluated the cytotoxic response of RKO cells with endogenous TR1 as well as cells where the TR1 was attenuated with siRNA to reactive oxygen and nitrogen in the form of H2O2, the oxidized lipid 4-HNE, or the NO donor JS-K (Figure 4).
Current technology of siRNA delivery relies on pharmaceutical dosage forms to route maximal doses of siRNA to the tumor.
Linear tetraspermine (MW 2581.82) and dendritic tetraspermine (MW 2625.87) naturally have a higher molecular weight compared to linear bisspermine (MW 1299.40), which affects the ability of the polymers to interact with siRNA and to form polyplexes.
The vector originality is based on the fact that, in addition to the siRNA molecule, it contains two components: 1) a cationic liposome that auto-associates with the siRNA to form particles called "lipoplexes" and, 2) an anionic polymer which enhances the lipoplex's efficiency.
CS combined with PLA at the optimal ratio was able to form positively charged, nanosized polyplex with siRNA and successfully delivered siRNA to the cells in vitro.
This cathepsin B-biodegradable polymer interacts electrostatically with the siRNA to form a nano-sized polyplex stable in plasma.
However, SWNTs could efficiently facilitate the coupling of siRNA to form siRNA∶SWNTs complexes and carry siRNA into K562 cells, significantly knocking down the expression of target gene.
These cationic polymers were used to condense siRNA molecules in the form of polyplexes.
The ability of oligospermines to condense siRNA and to form polyplexes with defined structures was therefore evaluated here.
