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In the worst case (rab3 library, moderate transcript initial abundance), with respect to transcripts of the same initial abundance, we observed five-fold less representation in the library for the tester/driver commonly expressed transcript when compared with the tester-specific transcript (Table 1).
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(A ) Density plot of the relative length of the primary ORF in lncRNA_ribo and codRNA with respect to transcript length.
This suggested that we have assembled and captured an intact transcriptome with respect to transcript integrity and trancriptome completeness.
With respect to transcript levels, a large amount of microarray data is publicly available for Arabidopsis thaliana, a model plant.
Both Cdk2 and Cyclin D were down-regulated in response to infection with P. gingivalis with respect to transcript levels and protein levels.
We performed meta-gene analysis (Shin et al. 2009) to evaluate the distribution of H3K9me2 marks with respect to transcript level in him-8 gonads.
Compared to transcriptome shotgun sequencing (RNA-seq), Tag-seq does not reveal full transcript sequences, but has the advantages of being strand-specific and unbiased with respect to transcript length.
The relative length of the ORF with respect to transcript length did not seem to be a strong predictor of ribosome association, as it did not help distinguish lncRNAs associated with ribosomes (lncRNA_ribo) to those not associated with ribosomes (lncRNA_noribo).
AFLPinSilico was used to compare the efficiency of enzyme pairs and their combinations with respect to transcript coverage, fragment size, and the proportion of 3'-untranslated regions [ 5, 18].
A recent transcriptomic study on the Mediterranean mussel M. galloprovincialis also showed significant tissue-specific differences among four tissues with respect to transcript expression profiles in metabolic pathways [ 11].
Notably, HD-zip (XM_002271656.2, a homeodomain associated leucine zipper protein) negatively correlated with both VviHDR and VviUGT85A2L4 (VviGT14) levels with respect to transcript accumulation but was significantly up-regulated in the grapes of the GT region relative to the CL grapes at the E-L 35 stage.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com