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Transformation of these PCR products yielded declining number of recombinant colonies with respect to the insert length.
For instance, column c i −3, i −2 contains three types of bases: an null base which we denote as "d" (stands for deletion with respect to the reference), an inserted base of "AT", and an inserted base of "C".
Hence, the tails of each partial alignment generated by SSW often contain mismatches with respect to the reference or inserted sequence.
This algorithm permits the use of a more lenient Smith-Waterman score, since eventually the junk portion of alignments (with respect to the reference genome or inserted sequences) will be trimmed off.
The categorization of sequence reads with respect to the expected cDNA insert structure reflecting cDNA library construction protocols is a complex procedure due to the numerous variations that can be made in the molecular biology steps employed for cDNA library construction.
Similar calculations were made to obtain the comparison matrices of the criteria (using opinions inserted in Table 5), the comparison matrices of the alternatives with respect to the other criteria (using opinions inserted in Table 6) and their RI values.
An increasing number of longitudinal fins were inserted with respect to the fluid flow from the second to the fourth configurations.
The location of the inserted L4-cassette with respect to the PBi promoter was confirmed by PCR from genomic DNA using primers specific for the promoter and for TPL leader 3. Genomic DNA was isolated from cells using Tri-reagent (Sigmaccordinging to the manufacturer's instructions.
The tagged proteins were expressed in transfected LLC-PK1 kidney cells and tested for transport activity, and the polarity of inserted tags with respect to the plasma membrane was determined by immunofluorescence in intact and permeabilized cells.
In the adult human brain tau is expressed in six isoforms that differ with respect to the number of N-terminal inserts and microtubule binding repeats [2].
This new MCS was optimized to allow directional cloning whatever the orientation of the cDNA since sites for compatible enzymes (e.g. BamHI and BglII) have been inserted quite symmetrically with respect to the center of the MCS.
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