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For optimal results, it is also important to follow the recommendations of the chosen de novo assembler with respect to insert length of the sequencing library, minimum coverage of the target genome with PE reads and other recommendations.
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It was also counterbalanced with respect to which insert was rewarded in each pair.
Transformation of these PCR products yielded declining number of recombinant colonies with respect to the insert length.
These results indicate that parasites of clones derived from the transposase parasite line are not homogeneous populations with respect to piggyBac inserts but they consist of mixed populations of parasites containing inserts in different chromosomes.
For instance, column c i −3, i −2 contains three types of bases: an null base which we denote as "d" (stands for deletion with respect to the reference), an inserted base of "AT", and an inserted base of "C".
The categorization of sequence reads with respect to the expected cDNA insert structure reflecting cDNA library construction protocols is a complex procedure due to the numerous variations that can be made in the molecular biology steps employed for cDNA library construction.
Hence, the tails of each partial alignment generated by SSW often contain mismatches with respect to the reference or inserted sequence.
In particular, α1β2γ2-mediated currents showed a pronounced fast component with respect to α3β2γ2 (Fig. 4A insert).
An increasing number of longitudinal fins were inserted with respect to the fluid flow from the second to the fourth configurations.
The orientation of ligated inserts with respect to Sp6 and T7 promoters was determined by direct sequencing on an ABI 3100 capillary sequencer using the BigDye terminator reaction chemistry (Applied Biosystems, Darmstadt, Germany).
This algorithm permits the use of a more lenient Smith-Waterman score, since eventually the junk portion of alignments (with respect to the reference genome or inserted sequences) will be trimmed off.
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