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C57BL/6 mice were injected with ID83 i.m. three times two weeks apart with adjuvant doses of 1, 5 and 20 µg of GLA-SE or MPL-SE along with relevant controls, including antigen with SE alone.
To determine the effect of GLA on adaptive immune T cell responses, mice were injected with ID83 i.m. three times two weeks apart with adjuvant doses of 1, 5 and 20 µg of GLA-SE or MPL-SE along with relevant controls, and splenocytes were collected from mice ten days after the third immunization and incubated with or without ID83 in vitro for 72 h.
Overall, immunized AD brains had an apparent reduced vascular density compared with relevant controls.
Data (for this and subsequent MTT experiments) was plotted as percentage viability compared with relevant controls.
Perform repeat variant test(s) on the fetal DNA, along with relevant controls, possibly with different DNA concentrations.
Conversion of butyric acid to butanol by the engineered E. coli (pQE-adhE2/ptb/buk) strain was tested with relevant controls.
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Among the 50 genes with the highest M-values (Table 2), there are five transcriptional regulators, one sigma factor and one anti-sigma factor, which indicates that heat shock response is a complex system with relevant control at the transcriptional level.
To further explore the association of NRBP1 loss in cancer, we performed expression analysis on a wide variety of human tumour types (including leukaemia, lymphoma, colorectal, breast, brain, oesophageal, renal cell, prostatic and lung cancers) and found significantly reduced NRBP1 expression levels in many of these tumour types when compared with relevant control tissues (P<0.01; Figure 6A).
The underlying cause of this defect in Nmnat2gtE/gtE embryos is restricted axon extension (Gilley et al., 2013); therefore, to obtain a quantitative measure of rescue by SARM1 deficiency, we compared neurite outgrowth in SCG explant cultures from Nmnat2gtE/gtE and Nmnat2gtE/gtE; Sarm1−/− embryos with that of relevant controls.
On the other hand, mitochondria isolated from the tom70 knockout strain have levels of Tom20 similar to the control strain, but have much reduced AOX levels compared with the relevant control, consistent with the results in Figure 2B for 96C10.
Cultures of adult fibroblasts were transfected as described above with CCT-eta/CCT-beta siRNA (or the relevant controls), then stimulated with PDGF treatment.
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