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An initial coding framework was developed with reference to the transcripts, the original study protocol, the research question and the literature underpinning the intervention.
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In each cell we then summarized the participants' beliefs or strategies, with reference to the original transcript excerpts.
The peptide sequence alignments were back translated to nucleotide sequences, and the exon boundaries were delineated with reference to the human transcript annotations.
Next, codes were organised and grouped together into initial themes/patterns emerging from the data, with repeated reference to the transcripts.
We had found the same categories and there was only slight disagreement on two or three of the transcripts, and those differences were resolved through discussions and with reference to the total material in the transcripts [ 15].
Disagreements were resolved through discussions, with reference to the total material in the transcript [ 22].
The mRNA level was normalized with reference to the amount of housekeeping gene transcripts (GAPDH mRNA).
The increment for the GP PAH-RHD transcript was higher than the one for the GN PAH-RHD transcripts with reference to the beginning of the experimentation (P < 0.05).
After 6 months of incubation, the vegetation of N spiked sediments determined an increment, respectively, of 36.2 in rhizospheric) and 40.5 in bulk) portion in the fractional copy number of the GN PAH-RHD α transcripts with reference to the beginning of the experimentation.
The relative expression levels of the transcripts were calculated with reference to the housekeeping gene myosin (Genbank AC: 486090G09.x1).
To elucidate the molecular basis underlying volatile biosynthesis associated with the indirect defenses of elm in response to egg laying, we compared the different treatments with reference to transcripts involved in terpenoid metabolism.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com