Recombination was very robust, with EGFP recapitulating the entire CreERT2-positive domain of all double transgenic embryos.
These address the training of specific abilities, e.g. swallowing in patients with dysphagia, recapitulating lessons from speech or language therapy, or transferring from bed to the chair.
Interestingly, micromolar concentrations of PCB95 had no net effect on dendritic growth compared with controls, recapitulating the inverted dose-related effects of developmental A1254 exposure on dendritic growth in vivo.
We used G & G to assemble just those Rmaps (2,448,748) that escaped assembly within the iterative assembly pipeline, which yielded 1,500 optical contigs, with most recapitulating those constructed by iterative assembly.
In general, there is no perfect model as all models have implicit strengths and weaknesses, with none faithfully recapitulating the entirety of what occurs in situ.
With respect to recapitulating the CNS in vitro, there are limitations to the above methods.
A subset of genes (Ccr5, Jak3, Litaf) was confirmed at the level of protein expression, with protein levels recapitulating changes in mRNA expression.
The myofibroblasts are very closely associated with glandular structures, recapitulating the close relationship found between pericryptal myofibroblasts and normal colonic columnar epithelial cells.
Despite some of these limitations, particularly with regard to recapitulating dominant mutations and missense mutations, the techniques currently available have been used with great success to replicate several aspects of some MNDs, as discussed in detail below.
In the case of parvalbumin β, results of FEATURE coupled with dynamics recapitulated the behavior of the protein's Ca2+ binding sites with and without synthetic mutations (PDB IDs 1B8C and 1B9A, respectively).
