Drizzle more of the herbed olive oil on top and sprinkle with Parm.
I add in a bunch of Frank's and top it with parm and a bunch of packs of shakey pepps that were lefties from 'Za delivery.
At Portland's best sandwich shop, those hand-cut fries are loaded with Parm, herbs, peppers, and scraps from whatever house-cured pig they have chilling around.
This could be in accordance with PARM-1 expression in the hearts during normal developmental stages.
Given that the authors only give qualitative monomeric structural comparisons with ParM as evidence for the proposal that MreB dynamics are driven by dynamic assembly, I am skeptical of their conclusions that MreB operates through a dynamic instability mechanism.
In contrast, the fluorescence change obtained with ParM (D63C/K216C/C287A) was <3-fold with both tetramethylrhodamine isomers and this could not be improved by further purification of the labeled proteins.
From the ΔCcMreBdh co-crystal structures with ADP and AMPPNP, it is clear that the active site could well accommodate a guanidine instead of an adenine as the major contacts are with the ribose and phosphate moieties of the nucleotide, a feature, again, shared with ParM (Gayathri et al., 2013).
Garnish with vegan parm, if desired.
Another practical consequence of the weak nucleotide binding is that ADP contamination in ATP would be less problematic than with MDCC-ParM.
With regard to the second segregation system, StbA and StbB of pEntH10407K exhibit 99.1 and 99.1% amino acid sequence identity with StbA (ParM) and StbB (ParR), respectively, of R100, and a cis-acting site (parC) (90.4% DNA sequence identity with the corresponding sequence in R100) is present upstream of stbA on pEntH10407K.
