Finally, contigs longer than 2000 nt were chopped into 1999 nt with 1899 nt overlapping segments to accommodate a requirement of the Newbler assembler.
The mitochondrial noncoding control region (D-loop) was sequenced by PCR of four overlapping segments, with the use of forward and reverse M13-tagged primer pairs.
Our goals is to identify not only recurrent aberrations in many samples (primary events), but also rare, secondary aberrations that might be obscured by complex and overlapping segments with primary events.
In 68 % of patients, the LCX may run inferior to the CS, as a result of which it is located between the CS and mitral valve annulus, with an overlapping segment >30 mm in 17.58 % of these patients.
For any other segment (call segment C), if the modulation frequency range at least 80% overlaps with that of segment A or segment B, the segment C is grouped with that overlapping segment; otherwise, the segment C is omitted for the grouping procedure.
The 129 F4 and 10G1 clones contain an insert of about 37150 bp and 107473 bp, respectively, with an overlapping segment of 5201 bp [[ 16]].
To verify the presence of rare, relatively highly penetrant CNVs that could be responsible for a proportion of the schizophrenia phenotype, we defined as rare those CNVs with a ≤80% overlapping segment with a CNV previously described in the DGV (release 18v6; http://projects.tcag.ca/variation/).tcag.ca/variation/
The Atlantic herring sequence was obtained in two overlapping segments using #2 sense with #3 antisense and #3 sense with #2 antisense.
MNO: Matched nonoverlapping segments; number of nonoverlapping segments that are found in the targets transcript sequence; with a perfect match MNO = PNO; with no match MNO = 0. MOS: Matched overlapping segments; number of overlapping segments that are found in the targets transcript sequence; with a perfect match MOS = POS; with no match MOS = 0.
These segments were identified with a program written in Perl (scripts are available in the Supplementary Material), where segments with the same start and end positions between twins, as well as overlapping segments, were compared.
