The models were developed in the following equations (Eqs. 3, 5, 3) with only significant coefficients (P < 0.05): Table 5 Box Behnken design matrix along with the experimental (exp).
Among the four models that included additional predictors, M2 with form effects indicated the worst model fit, whereas M4 with only significant item position effects indicated the best model fit based on both AIC and BIC.
For both men and women, effects on RER were trivial-small with only significant differences between 0 and 10% BWS at 16.1 km·h−1 (p = 0.02) for men and 20 and 30% BWS at 16.1 km·h−1 (p = 0.04) for women.
To focus on genes with only significant changes in expression levels, we identified genes with a ten-fold or greater change in expression levels.
No significant effects were found for RU 28318 and RU 28318/stress interaction, with only significant effect of stress (F1,12 = 20.91; p<0.001) (Fig. 3D).
We then resumed the standard model simplification approach, removing least significant main effects from the fifth model (i.e., the one that contained only the five main effects) until we were left with the minimum adequate model (i.e., one with only significant terms).
