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A curiosity is that Illumina fails to identify robustly the chromosome 13 arm gain in the MT3 cell lines, suggesting an issue with the normalization applied by BeadStudio, but the main concern is the Nimblegen platform, which fails to spot some large aberrations in tumours T7195 and T7214.
Better results are obtained with T-quantile normalization applied separately on the channels or Tukey's biweight scaling.
The 3rd letter (column 2) specifies whether between-array normalization was performed (i.e. between-sample quantile normalization of M and U separately), with 's' indicating between-array normalization applied to Type I and Type II probes separately, 't' indicating between-array normalization applied to Type I and Type II probes together and 'n' indicating no between-array normalization.
Because we believe that the feature normalization applied to the raw modulation spectrum features yielded the best performance since it conforms with the mathematics in Lasso, we applied the same normalization to the LDA-transformed features.
Control spots were removed and loess normalization applied.
Total intensity normalization and LocFit normalization were applied with housekeeping genes as controls.
Log2 transformation, Lowess normalization, and quantile normalization were applied to normalize within and between microarrays.
The Raw fluorescence intensity values were normalized applying quantile normalization.
Splitter with no normalization was applied to the TRE ChIP-chip dataset with a SD of 2.35, MINRUN of 2 and GAPMAX of 488.
A varimax rotation with Kaiser normalization was applied to the solution to minimize the number of variables that have high loadings on each PC and to simplify the interpretation of the PC.
All indicators entered into a correlation matrix, and a varimax or thogonal rotation with Kaiser normalization was applied.
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