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There is a circular problem with normalising a target gene with reference genes that should be normalised themselves prior to target gene normalisation [ 9].
This is likely to have arisen from the high potential for small variations in the reaction setup with this method and the problems associated with normalising to a housekeeping gene [ 53].
The chip was primed in HBS-N (10 mM HEPES, 150 mM NaCl, pH 7.4) and normalised with normalising solution (GE Healthcare).
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"Any EU government should keep in mind that its not about normalising with a government, it's about normalising with a people who deserve to live in dignity like any people in the world".
Langhoff works with women on "normalising" some productive worries, while recognising unproductive worries and the negative cycles they create.
Pressure coefficients are determined by normalising with the wind dynamic pressure at the reference position.
"All Arab-passport holders entering any part of historic Palestine (67 or 48) on an Israeli visa are normalising with Israel.
MIBI was used to determine tumour uptake and in vivo functional imaging by normalising with whole-body region counts.
The relative changes in the amount of transcripts in each sample were determined by normalising with the β-actin mRNA levels.
We considered at least one of the following inclusion criteria: (i) proteins with normalised spectral count coefficient of variance <35% and FC ≥1.5 or ≤ −1.5; (ii) proteins giving VIP > 1 after PLS-DA.
The one-week treatment with SSR240612 normalised the higher production of O2− in glucose-fed rats to control levels.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com