Sentence examples for with normal sera from inspiring English sources

Substantial apoptosis was detected in EPCs after culturing in the presence of scleroderma sera compared with normal sera.

The brucellacidal activity of PMN is promoted by opsonization with normal sera [44] but it seems that the negligible complement binding displayed by B. abortus (Figure 4 A) which is related to the poor complement binding of its LPS [4] would be a limiting factor.

Eight pleural effusions from lung cancer patients gave positive signals (>two-fold signals in at least two different titres compared with normal sera of 48 donors).

Parallel control slides were prepared either lacking primary antibody or lacking primary and secondary antibodies, or were stained with normal sera to control for background reactivity.

For immunohistochemistry, sections were first washed with PBS + 0.2 % Triton X-100, blocked with appropriate normal sera for 1 h and incubated with the primary antibody overnight at 4 °C.

The background seen on the membrane incubated with the normal sera was considered nonspecific reactivity with phage proteins.

After heat-induced antigen retrieval, the slides were blocked with 10% normal sera (Jackson ImmunoResearch) before incubating in primary antibody (1° ab, anti-nitrotyrosine (Millipore)) and CSE: anti-CTH (Abnova), anti-CBS (Santa Cruz Biotechnology), anti-MPST (Sigma), anti-adipophilin (Progen), anti-eNOS (BD), and anti-HO-1 (Abnova).

In the corresponding negative control section, the primary antibodies were either omitted or replaced with appropriate normal sera.

Binding to non-arginine containing regions might account for the 'background', and hence the apparent loss of disease specificity seen when immunoblotting with the normal sera in our study, and the non-RA sera in that of Saulot and colleagues.

A total of 655 lung cancer (LC) sera (476 were from patients with non-small-cell lung cancer, 165 with small-cell lung cancer, 1 lung sarcoma and 13 of unknown histology) were compared directly with 655 normal sera, which were analysed as controls.

Most importantly, the molecule was not reactive with IgG in normal sera and, hence, could be employed in immunoblots or ELISA to specifically detect antibodies in schistosome-infected patients.