Each of the fitted models was compared to one with no breakpoint; the models with the breakpoint showed highly reduced AIC values.
Large inter-marker regions with no breakpoint produce large negative residuals, while small inter-marker regions with a breakpoint produce large positive residuals.
This enrichment of microhomology was also found when the analysis was repeated using only deletions with Alus at their breakpoints, as well as using only deletions with no Alus at their breakpoints, indicating that microhomology is not specifically associated with the Alu elements.
For disk diffusion, Mueller-Hinton agar, 120-μg gentamicin disks, and 300-μg streptomycin disks with breakpoints of no zone for resistance and ≥10 mm for susceptibility gave the best sensitivity and specificity if results for strains with zones of 7 to 9 mm are considered inconclusive, indicating that a broth or agar test should be performed to determine susceptibility or resistance.
In contrast, we did not find correlations between breakpoints and ribosomal protein genes, small nucleolar RNAs, small nuclear RNAs, and Spo11 hot spots (supplementary table S6, Supplementary Material online), features with no anticipated association with breakpoints.
The current National Committee for Clinical Laboratory Standards (NCCLS) guidelines make no distinction between these two interpretations of MIC, with clinical breakpoints used to characterize most antimicrobial agents, including the fluoroquinolones.
Cases with certain breakpoints collected in the Zurich Cytogenetic Database were transferred to ECARUCA.
Two of the five (40%) were A/G/J mosaics with common breakpoints.
The threshold-like transitions with distinct breakpoints were more apparent in C-ΣQ patterns for all solutes.
In one patient, MYC was translocated with multiple breakpoints suggestive of convergent evolution of independent rearrangement events in different subclones (Fig. 1b).
