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However, in the downstream processing, prevailing bottlenecks have not been well addressed with new methodological concepts.
Spatial perspectives to ecological research have proven beneficial (e.g. Liebhold and Gurevitch 2002), but they have also presented researchers with new methodological challenges (e.g. Legendre and Fortin 1989; Legendre et al. 2002).
However, there is currently significant research activity in this area addressing these problems, with new methodological approaches displaying a better capacity to specifically distinguish the true co-evolutionary information [12] [14].
The recent availability of huge amount of sequence data for a wide range of organisms, together with new methodological developments of in silico mining of microsatellites, have tremendously increased the characterization of these markers [ 5], and will certainly catalyze the study of genomic distribution of microsatellites in eukaryotes.
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We thus expand our analysis with a new methodological approach, combining the use of prospective and statistics techniques.
As with any new methodological developments there will almost certainly be opposing views.
For this analysis we combined parts of an established process analysis method by Pomberger and Gerken [ 33– 35] with semi-structured interviews, feedback loops and classification methods in a new methodological approach with 14 steps (Fig. 1).
Integrating these new methodological developments with important theoretical insights holds major promise for the future of biogeography and charts a pathway connecting past and future discoveries.
Whole genome data both require and permit new methodological approaches to deal with biases that lead to strong conflicts among individual gene trees [ 32].
Nevertheless, despite recent promising positive clinical trials, new methodological approaches for therapeutic protocols with adaptable outcomes to assess progression are still needed.
New methodological approaches should be developed to deal with the study design inconsistencies and the knowledge gaps identified across this review.
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