For denatured hybridization, the gel was denatured with denaturing buffer containing 0.5M NaOH and 1.5M NaCl for 20 min, and neutralized with neutralization buffer containing 0.5M Tris pH7.0 and 3 M NaCl for 20 min. The denatured gel was then hybridized with C1-3 probe described above.
Immunoprecipitates were eluted from Dynabeads using glycine-HCl (pH 2) treatment for 10 min and were subsequently neutralized with neutralization buffer (0.5 M Tris HCl, 1.5M NaCl).
The membranes were neutralized with neutralization buffer (0.5 M Tris-HCl, pH 7.4; 1.5 M NaCl) for 3 min, washed with 2× SSC, and cross-linked by UV cross linker (Stratagene, La Jolla, CA).
In vitro, neutralizing activity correlated with avidity and with neutralization rate constant, a measure of on-rate.
EV71vac elicited mouse antibody responses that cross-neutralized different EV71 subgenotypes (B1, B3, B4, B5, C2, C4 and C5) with neutralization titers ranging from 1/64 to 1/1280, but failed to neutralize CVA16.
Accordingly, the net effect of therapeutic neutralization of APRIL concomitant with neutralization of BLyS might be beneficial, harmful, or neutral in the context of the ongoing autoimmunity of SLE.
Extended X-ray absorption fine structure (EXAFS) spectra differed slightly with neutralization level in nominally dry ethylene methacrylic acid copolymer ionomers neutralized with zinc.
Overall, all viruses were neutralized by pooled sera from the 1997 and 2004 outbreaks with neutralization titers ranging from FRNT80 = 80 1280.
Neutralization of lethality in mice correlated with neutralization of in vitro coagulant activity in human plasma.
The identified minor minerals were reactive sulphide and sulphate, which promote generation of ARD and carbonates with neutralization property.
The aspect ratio generally increases as the neutralization level increases, except for Na+ ionomer nanocomposites with neutralization levels >50%.
