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Last year the award was won by Armand Marie Leroi with Mutants: On the Form, Varieties and Errors of the Human Body.
Mouse strain background had a significant effect on SWD, with mutants on the C3HeB/FeJ strain showing a higher incidence than on C57BL/6J.
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This seems like a likely explanation in this current comparison, but this might change when we deal with mutants and transgenes maintained on an outbred strain.
In addition to the gene expression arrays, we also studied the impact of infection with recombinant NYVAC mutants on the maturation of DCs.
Previous infection studies with TRI5 knockout mutants on wheat revealed that the trichothecene deficient mutant is still able to infect the inoculated wheat spikelet like wild type [ 9, 11- 13].
Further work investigating Cdc42 interaction with APC and its mutants on SW480 cells will likely reveal novel insights into the biology and cancer-causing abilities of these proteins.
We created a random insertional mutants library of U. maydis L8 strain by the Agrobacterium tumefaciens-mediated transformation (ATMT) technique, and screened approximately 20,000 T-DNA insertional mutants on plates with glycerol as the sole carbon source.
This result is qualitatively in agreement with the fact that icd mutants on S. meliloti are symbiotically ineffective [ 28].
These effects were more pronounced upon mutation of Cys and Cys alone or in combination, correlating with the impact of these mutants on the redox state of ERp57.
(2008) adopted global transcription machinery engineering (gTME; see section on 'Novel techniques' below) to select mutants on medium with xylose as the only carbon source and were able to identify an isolate with improved xylose fermentation.
The transposon reaction of plasmid pMM5Z (see Table 1) was electroporated into DH5α using a BioRad Gene Pulser according to the manufacturer instructions, selecting for insertion mutants on plates with chloramphenicol and ampicillin.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com