Solanum acaule was thought to be a segmental allotetraploid with minor variants of a common A genome.
(B ) Similarly, the fraction of sites with minor variants above 1% increases over time at the least constrained positions (quartiles Q3 and Q4), while few sites in the most conserved quartiles (Q1 and Q2) are polymorphic.
SNPs were classified into frequency categories: those with a minor variant frequency less than 0.1, those with a frequency between 0.1 and 0.2, and those with a frequency greater than 0.2.
The formulas below describe the decomposition of the entropy for a population of haplotypes (in a given window), into a total entropy and a conditional entropy associated with a minor variant - the conditional entropy associated with the major variant would also be a part of this decomposition but is not used for our score.
Because of this property of TBP-binding sites, which was proven empirically by many research groups [ 74, 123, 124], leptin production in a patient with the minor variant -35g of rs201381696 may be proportionately lower (in each tissue expressing the LEP gene, regardless of its tissue specificity) in comparison with a person carrying the variant WT.
Using mutation specific HRM assays with a 10% minor variant detection limit, we confirmed heterozygosity of the variants in the P-Tu sample as well as the higher passages of P-STS sample and the absence of a ≥ 10% subpopulation in the L-STS p49 and H-STS p42 cell lines (Additional file 1: Figure S2).
Regardless, these data demonstrate that BCO allele-specific sequences interfere with tag-antitag recognition, indicating minor variant detection with the multiplex OLA was hampered by impaired BCO binding and assay variability was due in part to variability in BCO binding (BCO mimic Bmax coefficient of variation = 0.33 for the data set).
Furthermore, the analysis of the different gallstone carrier subgroups revealed a significant association of the minor variant with gallstone susceptibility in the normal weight group (p = 0.00754, OR = 2.90).
Repeatedly with only minor variants the same bygone".
The larger the difference between entropies, the more conserved (longer) the haplotype that carries the minor variant, consistent with a recent selective sweep.
