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The preparation of cartilage explants was performed according to our previous report with mild modification [ 17].
DNA was extracted by using Lee technique [ 21] with mild modification.
The method to detect serum complement factor H CFHH) was the same as previously described [ 17], with mild modification.
The preparation of primary cultures of MCs was performed as described previously [ 25, 26] with mild modification.
Mutation analysis of TP53 exons 3 9 was performed by PCR and direct sequencing according to previous reports with mild modification.
The ASXL1 exon 12 until the stop codon was amplified by three pairs of primers and sequenced by another six internal primers, as described by Gelsi-Boyer et al., with mild modification.
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Causes of death were classified on the basis of maternal, obstetric, fetal and neonatal characteristics identified in the linked registry data, according to the NICE classification [ 23], with a mild modification of the unexplained asphyxia category (Table 1) based on our previous modification [ 20].
Imaging results were reviewed with the referring physician, who then determined whether a modification in the management plan was needed: 1/6 no change, 2/6 mild modification (e.g., additional diagnostic test ordered) and 3/6 significant modification.
The proof of which is a mild modification of the proofs in [5, 9].
The minerals were treated with mild acid before modification using silver nanoparticles sources and titanium-oxide nanoparticles.
However, patients on OxFU+cetuximab with mild renal impairment required more dose modifications compared with patients of CrCl >80 ml min−1 on the same regimen.
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