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The infrared data provided an opportunity to see if earlier generations of stars - ones with low abundances of heavy metals - correspond more closely to current theories of dust formation in exploding supermassive stars.
Prior to the accidental experimental discovery of a few scrambled transcripts with low abundances from the DCC gene in the human cell line (Nigro et al., 1991), a study using an electron microscope had already reported that RNA could be present in a circular form in the eukaryotic cell cytoplasm ten years ago (Hsu and Coca-Prados, 1979).
Conventional PCR often is not sensitive enough to amplify amoCAB genes from natural habitats with low abundances of AOB (Beman and Francis 2006; Leininger et al. 2006).
Species with low abundances at the range margin have low, if not null, migration potential projections, irrespective of forest density in the area into which the species is projected to migrate.
Other abomasal species belonging to the subfamily Ostertagiinae (Ostertagia ostertagi, Os. leptospicularis, Spiculopteragia spiculoptera morph spiculoptera, Te. pinnata and Te. trifurcata) were also present but with low abundances and prevalences (Table 5).
A nested PCR approach to amplify environmental amoCAB sequences has recently been established, providing an increase of the sensitivity in detecting amo genes in samples with low abundances of AOB (Junier et al. 2009).
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The results revealed complex pyrene degrading communities with low abundance of individual degraders instead of a limited number of abundant key players.
As reported by Gavin et al. for the protein complexes data used in this study, their tandem affinity purification procedure favoured more abundant proteins, but, nevertheless, they still detected some proteins with low abundance [ 9].
However, the human genome is well annotated with abundant EST evidence, allowing evaluation of HMMSplicer's performance on transcripts with low abundance, alternatively spliced junctions, and non-canonical junctions.
For this reason, synthetic peptides were used as antigens for mAb production that aimed at the AIV proteins with low abundance or poor immunogenicity in the virus particle.
In this work, we present a five-layer microfluidic system for parallelized, rapid, quantitative analysis of RNA templates with low abundance at the single-cell level.
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