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The first PRM with large-scale affinity data available is the PDZ domain (Stiffler et al., 2007).
Cellulose is an ideal matrix for large-scale affinity purification procedures.
GST-Arl5 T30N and GST-Arl5 Q70L were produced in Escherichia coli BL21-GOLD (DE3) and used for large-scale affinity chromatography of cytosol prepared from adult fly heads.
Large-scale affinity chromatography was performed with pellets from 0.5 l bacterial cultures and 1 × 10 HeLa cells.
Additionally, molar amounts per mL of transcription reaction were estimated for the C.b. variants prepared using the large-scale affinity purification.
A recent large-scale affinity column proteomic analysis has identified the Sec23/24 complex as interacting with 14-3-3 14-3-3 14-3-3io et al. 2004).
Between 10 15 mg of nuclear extract were used for each large-scale affinity purification.
In yeast [ 3- 8], human [ 9, 10], Drosophila [ 11] and C. elegans [ 12], genome-wide Y2H screens and large-scale affinity purification/mass spectrometry studies have been performed.
Israel responded with large-scale "retaliation raids".
It's that technology with large-scale video.
Then why stick with large-scale extractions?
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